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Collection stabilization and transport of saliva samples

Transport of saliva samples and other cares

Collection, stabilization, and transport of saliva samples are important now more than ever. And it is that since we live in the middle of a pandemic, care in the entire process of extraction and handling of samples requires greater neatness. Therefore, we need products that facilitate procedures. A job that companies like Real offer to professionals in the sector.

Collection stabilization and transport of saliva samples

Collection, stabilization and transport of saliva samples

Collecting samples for analysis requires precise conditions to prevent contamination. And is that any interference can condition the results. What cancels the test and makes the procedure unfeasible.

To avoid this situation, the Real company has launched REAL SALIVA VIRAL Sample Collection Kit. A product that provides an all-in-one procedure. And to be able to do with it a fast and safe collection; as well as to ensure its stabilization and the transport of one-milliliter saliva samples. And all can be done at room temperature, as the product stabilizes the viral DNA and RNA.

This stabilization is possible thanks to REAL VIRAL Stabilization Solution. A product that efficiently inactivates viruses. What solutions for the handling of non-infectious samples, making possible a safe handling and shipment of all these tests.

One of the main advantages of this system is that the samples are kept at room temperature. In the case of DNA, up to one year. Regarding RNA, up to a month. In case they are subjected to low temperatures, the conditions change. And it is that if they are kept between -20 / -80 ° C they can be kept indefinitely.

Collection stabilization and transport of saliva samples

Advantages of the Real Saliva collection and stabilization system

The main advantages of the sample collection, stabilization and transport system developed by the Real company are the following:

  • Allows the handling of samples of one milliliter of saliva.
  • Inactivates microorganisms and viruses for easy and safe handling and transport.
  • It is a product that allows the isolation of human genomic DNA and viral DNA / RNA. A variety not offered by other similar products on the market.
  • As already mentioned, this product allows transport at room temperature. This means eliminating cold infrastructure elements that hinder and retain transportation by requiring more procedures and paperwork.
  • Makes it possible to preserve total DNA in samples, including viral DNA, at room temperature. But if a temperature between 4º-25ºC is kept, it will be possible to do so for a whole year.
  • In the case of RNA there are very interesting advantages. And it is that it allows total RNA to be preserved, including viral RNA, at room temperature. And if we do it in a cold system (those that are between 4º-25ºC can be kept for a whole month.
  • They are systems compatible with most of the DNA and RNA isolation methods found on the market. Although it is best to use REAL products. Both those offered as kits and those sold independently.
  • It is a product specially designed for health professionals. And its handling is very simple and intuitive.

 

So, if you are looking for a collection, stabilization and transport system for any of your saliva samples, at Durviz we have what you need. Do not hesitate, visit our website to expand this information and get in touch with us.

sperm separation. Durviz S.L.

Vitromed V-GRAD for sperm separation

If you are looking for products with which you can improve the research you develop in your fertility clinic, such as products with which you can carry out sperm separation, in Durviz we have everything you need.

It does not matter whether the work is carried out in the field of a laboratory or a research ed (clinical, university-research or veterinary in selective breeding). In all of them you can use it with total peace of mind and sure that the results you get will be very reliable. We only have first manufacturer brands, with all the warranty seals and having passed the respective controls of each of them.

 

Durviz products for sperm separation

In Durviz we distribute a lot of products with which we can carry out the separation of sperm, although one of the most popular is V-Grad from the company Vitromed.

This product is a sterile colloidal suspension of silica particles stabilized with covalently bonded hydrophilic silanes. That is, a density gradient, which allows to separate the seminal sample according to the ability of sperm to pass through different media with different density.

In this particular case V-Grad is concreted in a kit composed of two glass bottles of 12 milliliters and 40% of 80 milliliters each. V-GRAD is a product that carries endotoxin tested and that has a sperm survival test close to 80% in all cases in which it has been used during its study.
For better visibility during laboratory handling, this product is colored with phenol red. Which makes it easy to use at all times. It is a product that we can use in the long term, since the validity period of the sample that is handled is 18 months from the date of production.

Vitromed Quality

The efficacy of Vitromed products is widely accepted in the clinical sector. Hence it is one of the most prestigious of those that exist today. Not all producers can boast the following quality standards:

  1. As in all the products that we can purchase from the Vitromed brand, these are substances, tools and laboratory products made of medical grade polymers that are manufactured under clean room conditions, ensuring successful assisted reproduction.
  2. In addition, all of them have been sterilized by radiation according to the ISO standard. They have also been tested for toxicity prior to the release of the product on the market.
  3. All our laboratory products are tested for MEA and LAL. This is an added guarantee for the quality of the tests to which each of them have been subjected.
  4. It is a company that develops and manufactures micro tools, needles and many other clinically advanced laboratory articles used at all stages of assisted reproduction techniques (popularly known as ART). Hence, its research and development department includes experienced German scientists, opinion leaders and engineers who work as a team to develop quality IVF products. Minds that progressively output high-quality products that allow important advances in the field of assisted reproduction.
sperm separation. Durviz S.L.

If you are interested or interested in purchasing any of the products that we have in Durviz to carry out the separation of sperm, or any other of the Vitromed brand (we have many in our catalog that you can know online from your own home) contact us. We serve all of Spain and are exclusive distributors of the main companies in the sector.

Covid-19 quick test for IgG/IgM detection

You can now find covid-19 quick test for IgG/IgM detection in Durviz. An immuno lateral flow chromatographic assay for the qualitative detection of IgG and IgM antibodies against 2019-nCoV in human blood, serum, plasma or also in a lancet blood sample. This puts at our fingertips an effective method for rapid diagnosis of COVID-19 infection in the person undergoing the test.

And all this doing a comprehensive analysis of the composition of the elements in the blood. So the detection of COVID-19 IgM antibodies tends to indicate recent exposure to COVID-19, while detection of COVID-19 IgG antibodies indicate a later stage of infection. Information needed to perform epidemiological studies, as well as the degree of involvement the disease had on the person.
And it is widely accepted that IgM provides the first line of defense during viral infections, followed by the generation of IgG’s high-anity adaptive responses for long-term immunity and immune memory.
Covid-19 quick test

Covid-19 quick test: IgM efficiency

Immunochromatography is one of the most relevant immunodiagnostic techniques out there. Its main advantages are: the simplicity and speed of the test.
Y is that more and more applications are being made of this technique, both in the field of tests, because no reagents or additional instrumentation are necessary; and in the clinical field.
This action can be performed using a simple device developed to detect the presence (or absence) of a target compound in the sample (the matrix). Element that makes up this test. It consists of two components: an IgG and an IgM, which allow to detect the presence of IgG and IgM antibodies independently.
Its operation is simple and very effective since the region of the IgG detection line is coated by human anti-IgG, and when depositing the sample in the analysis area, the possible IgG anti 2019-nCoV present, react with colored particles coated with antigens of 2019-nCoV present in the cassette. The mixture migrates through the membrane by capillary and reacts with human anti-IgG antibodies in the test region reserved for IgG detection. If the sample contains IgG antibodies for 2019-nCoV, a colored line appears in the designated region of IgG as a result of this antigen-antibody bonding reaction.
Similarly, in the IgM detection line, human anti-IgM antibodies are attached, in the case of a positive sample for IgM from 2019-nCoV, the complex formed together with the 2019-nCoV antigens reacts with the anti-IgM antibodies and a colored line appears in the corresponding region.
If the sample contains neither IgG nor IgM, no lines will appear in the test areas for IgG or IgM, indicating a negative result. However, as a check to ensure that the sample volume used is sufficient and that the procedure has been successful, a third line appears in the control area.  
These types of tests are used for medical diagnosis and home testing, as well as in the lab.
Covid-19 quick test

Covid-19 quick test

A reliable and quality test

Given the importance of results in people’s lives and in the safety of their environments, special attention has been paid to test controls.
In fact, internal controls are included in the test. This is displayed on a colored line appearing in the control region (C) composing the internal control of the procedure. This ensures that the technique carried out is correct and that the sample volume used is sufficient. Control standards are not included in the kit, however, it is recommended to test with negative and positive controls as indicated by good laboratory practices to verify the test result.
DNA and RNA extraction kit. Durviz S.L.

NOW you can buy the DNA-RNA intron Extraction Kit at Durviz S.L

In Durviz we offer you the possibility of having units of the Kit for the extraction of DNA and RNA of the Brand Intron, one of the most prestigious in the sector and with the most proven efficiency.

This tool will allow you to easily and quickly perform analysis of various pathogenic samples infected by viruses or bacteria found in substances such as plasma, blood, serum, cellless bodily fluids, cells themselves, tissues, etc.

These products are now more than ever necessary in the daily activity of laboratories around the world. But it is also necessary against premium products that ensure that the results we get from these tests are reliable and allow us to have data with which to put strategies in the right way.

intron kit extracción ADN -ARN

DNA Extraction Kit – Intron RNA

The Intron Brand DNA and RNA Extraction Kit is one of the most complete on the market. Compared to other analysis systems, those that allow the extraction of ADRs and ARNs from this brand offer the following advantages compared to other options that we can find on the market:

– It is a system that uses a low concentration of caótropic salt and better lysis efficiency. Something that does happen with the conventional products that we have in the sector. Especially those that focus on total RNA extraction.

–It has a greater efficiency of lysis without additional additives, which means obtaining results with greater reliability since there is less chance of contagion or alterations in them.

– It is very effective in the stable extraction of DNA and RNA from various pathogenic samples.

– It employs for its proper functioning, a method without phenol and without the need to perform EtOH precipitation

– It is an appropriate system for the extraction of samples such as forensic medicine and diagnosis of diseases, among many other actions.

dna rna extraction

With all this, this kit is used with total efficiency for the extraction of DNA and total RNA that is present in samples of pathogens such as viruses and bacteria, even those found in plasma or anticoagulated blood, in serum, in cellless and cellless bodily fluids, as well as in pathogenic tissues, among many other alternatives. This makes it easy to induce lysis, and do so without using any additives.A goal that is achieved by this kit using substances such as mercaptoethanol. In addition, DNA and RNA extraction is faster and more efficient, as it is done by membrane columns. This means that DNA and RNA isolated through this analysis system can be used to perform clinical diagnosis of people and animals infected with diseases caused by viruses and bacteria. Like, for example, Coronavirus.

Thus, the main – but not the only – applications of this kit are as follows:

-Detection of pathogens 

-PCR or RT-PCR 

-PCR quantitative (qPCR, qRT-PCR)

-cADn synthesis

-Research of infectious diseases.

So, if you want to have top quality technology in your analyses, do not hesitate and buy this kit. You can find it in our product catalog and ask us for as many units as you need. We will take you to wherever you indicate with all the guarantees.

Remember that in Durviz we are specialists in the provision of laboratory material, a sector in which we stand out for having the exclusive distribution in Spain of several brands of international relevance.

COVID-19: RT-PCR Kit for detection of RdRP, E, N and RNaseP genes

There are many people who want to know if they are infected with COVID 19. And the fact that we can be carriers of the virus and not show any symptoms, is especially delicate when we work in sectors where we come into contact with many people hence the importance of having a COVID-19: RT-PCR Kit.

COVID-19: RT-PCR Kit

The RT-PCR KIT is an analysis kit that allows us to know if a person is infected with coronavirus. Remember that you have to be very scrupulous when selecting a virus with these characteristics since there are four genes in the Coronavirus family and you have to choose the test that best suits each one of them.

Each of the genes in the coronavirus family is known by the name of one of the letters of the Greek alphabet. In this case with the first four: alpha, beta, gamma and delta.

Corona alpha and beta viruses can cause disease in both humans and animals, while others, such as the gamma and delta coronaviruses, only infect animals.

Well, the new coronavirus (specifically named as COVID-19) belongs to the beta gene family and is one of the new infectious coronaviruses that infects the human body as a pathogen of massive pneumonia that occurred in Wuhan, Hubei (China ) in December 2019. A disease that months later has become a pandemic and affects the population of the entire planet, given the speed and ease with which it spreads.

Hence it is especially important to diagnose an infection quickly, as there are no vaccines or antivirals approved for prophylactic or therapeutic purposes. What can cause death in case, above all, that the infected person has diseases related to chronic respiratory tract.

COVID-19: RT-PCR Kit.

Well, to be able to detect it in time and catch the disease at its beginning, there is nothing better than to test people with symptoms (or who have been close to those who have had it). And to carry it out the best is to have the most reliable test on the market: the COVID-19: RT-PCR Kit, a test that is validated by the Carlos III Institute (to which the National Center for Microbiology belongs)

The guiding principle of this kit is that you can detect the new coronavirus using the RT-PCR probe method in real time, through the reaction of the specific primer and the fluorescent probe in the sample.

In addition, this test can also detect the RdRp gene and the E gene, markers for the detection of new coronaviruses, so it is also a new way of anticipating the infection of other similar genes. In addition, the N gene suggested by the US CDC is included. and the RNaseP gene.

Its intended use is using sputum that accumulates in the lower airways, bronchoalveolar lavage fluid (BAL), or nasopharyngeal smear (NS) and oropharyngeal smear (OS) collected simultaneously in the upper airways.

Single cell analysis with TAPESTRI from Mission Bio

Tapestri ™
Plataforma de precisión para análisis de célula única
Las enfermedades complejas evolucionan, por lo que comprender la variabilidad genética – incluida la coexistencia de mutaciones a nivel unicelular – es de vital importancia para que los investigadores clínicos rompan el ciclo respuesta al tratamiento, resistencia y recaída.
Más información

SARS-CoV-2 RUO & IVD Controls for QC & Assay Development

From assay development to routine quality control, SARS-CoV-2 research materials and IVD controls from Microbiologics combine precision with the convenience of user-friendly formats

More information

DNA-extraction-kit-from-saliva-at-Durviz

DNA extraction kit from saliva at Durviz

Saliva is a substance that can give us a lot of information about a person’s state of health. At Durviz we know how interesting and reliable the data that can be extracted from a person’s saliva is, which is why we have products that facilitate its analysis. It is very important to have first-class products that help us to preserve the saliva sample without any contamination..

For this reason, in our virtual store we have products such as the Real Saliva Collection and Transport, one of the most popular on the market. This kit provides a fast and safe all-in-one method for the collection, stabilization and transport of saliva samples at room temperature.

Its main characteristics are the following:

  • Easy collection, transport and processing of saliva samples.
  • Painless collection, and non-invasive (as opposed to other systems where samples are taken from patients).
  • Allows saliva samples to be stored for 2 years.
  • The saliva kept in this kit is preserved and ready to be sent.
  • It allows the subsequent isolation of DNA making it an element of excellent quality and integrity.

The operation of this kit is very simple and requires few steps, which speeds up the work in the laboratory. The first thing to do is to collect the saliva samples. This is done by simply spitting into the collection funnel that has been assembled with a collection tube. This eliminates almost 100% of the possibility of contamination.

After collecting 2 millilitres of saliva (container capacity), the content of the saliva preservation solution is added and mixed with the extracted saliva. Finally, the saliva collection tube is sent to the laboratory for further isolation and DNA analysis.

This is usually recommended with the Real Pure saliva kit, which follows the same protocol and
that you get unbeatable results. The saliva samples are kept stably for more than one year at room temperature and more than 2 years if these same samples remain within the following temperature range: -20ºC or -80ºC.

DNA-extraction-kit-from-saliva-at-Durviz

For best performance, the manufacturer also recommends the following for the
when saliva samples are collected and preserved:

1. It is advisable not to eat, chew gum, drink or smoke during the 30 minutes before the saliva sample is taken.
2. It is advisable to wash out the mouth with water and wait for 10 minutes.
3. It is better if the patient rubs his cheeks and the palate with his tongue before collecting the sample. This gesture is sufficient to stimulate the secretion of saliva.
4. Before starting to collect the sample, it is convenient to place the funnel in the saliva collection tube. Then just spit the saliva into the funnel until the liquid (not including the bubbles) reaches the 2 milliliter line in the collection tube. If there are excess bubbles, they should be removed by gently tapping the tube on a hard surface.
5. Remove the blue cap from the saliva preservation solution.
6. Add the saliva-conserving solution to the collection tube.
7. Close the saliva collection tube tightly to avoid accidents or contamination.
8. Shake for 15 seconds to thoroughly mix the products with the saliva.
9. Once this procedure is done, the saliva sample is now preserved and ready to be stored, shipped or processed.

For all these reasons, this new model of Saliva Collection Kit is one of the most popular on the market. And one of the most popular among laboratory professionals.

Tapestri missionbio

MissionBio: New Brand for DNA analysis

From now on people and companies who wish to purchase MissionBio branded products in Spain can do so through the Durviz. website. This entity will be responsible for, from now on, the exclusive distribution, for our country, of the products of the firm MissionBio. A highly valued brand in the field of single cell DNA analysis using Tapestri technology.

Tapestri technology is very useful for the study of disease behavior since in their development these infections mutate and it is necessary to know how they do it to improve in applications, their treatment and their resistance.

 

tapestri missionbio

To achieve this, MissionBio’s Tapestri system performs the following actions:

  1. Breaking mass limitations.

At this time, Mission Bio’s Tapestri system makes the average reading of conventional mass sequencing; average reading of conventional mass sequencing is performed; which means it loses the underlying genetic diversity in cell populations. To improve patient stratification, therapy selection and disease monitoring, we need information on the occurrence of mutations within each cell and this is what MissionBio offers us with this system.

 

  1. The industry’s first scalable, customizable single-celled DNA analysis platform.

Tapestri is presented as the industry’s first scalable and customizable single-celled DNA analysis platform. It should be borne in mind, at this point, that the Mission Bio Tapestri platform was developed to help advance precision medicine, as its application allowed for accelerated and accessible detection of genomic variability within and between cell populations.To do this, taking advantage of the patented microfluidic droplets, the platform unlocks access to DNA at the level of individual cells with a novel two-step protease workflow. Actions whereby this methodology provides flexibility for additional applications and customization capability in our work.

In this way, the Mission Bio Tapestri platform enables the advancement of precision medicine by enabling the rapid and accessible detection of genomic variability within and between mobile populations. Something that happens by taking advantage of the patented microfluidic droplet, the platform unlocks access to DNA at the level of individual cells with a novel two-step protection workflow.

With all this, this methodology provides flexibility for additional applications and customization capability.

And this platform has the following features:

  • SNV directed and accurate and so-called independent variants.
  • Single cell DNA performance up to 10,000 cells.
  • Simple workflow.
  • Easy-to-use bioinformatics software.
  • Fully customizable content.

How Tapestri platform works

tapestri missionbio

The Tapestri instrument divides thousands of cells into individual droplets, which act as mini reaction tubes that allow thousands of individual reactions simultaneously.

In this way, cell lysis and protease digestion then continue outside the instrument allowing access to DNA and proteins for downstream amplification.

In detail, the steps in which the work of this MissionBio system is developed are as follows:

Step 1: The Tapestri instrument divides the cells into droplets along with a mixture of protease enzymes. Cell lysis and protease digestion are then performed, outside the instrument, allowing access to DNA for downstream amplification.

Step 2: Cellular lysate is combined with pearls, primers and reagents with barcode. Specific regions of interest are amplified by labeling each enlargement with a unique cell barcode, preserving the cell’s identity and mutation profile.

Step 3: After running your sequencer, the system continues to use a subsequent analysis and visualization software.To do this, Mission Bio provides dedicated bioinformatics support to help you discover biological and clinical knowledge

So, if you want to improve research based on single-cell DNA analysis, trust MisssionBio systems. In this way you can obtain the most reliable results and be able to develop very efficient strategies against different diseases.