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Transport of saliva samples and other cares
Collection, stabilization, and transport of saliva samples are important now more than ever. And it is that since we live in the middle of a pandemic, care in the entire process of extraction and handling of samples requires greater neatness. Therefore, we need products that facilitate procedures. A job that companies like Real offer to professionals in the sector.
Collection, stabilization and transport of saliva samples
Collecting samples for analysis requires precise conditions to prevent contamination. And is that any interference can condition the results. What cancels the test and makes the procedure unfeasible.
To avoid this situation, the Real company has launched REAL SALIVA VIRAL Sample Collection Kit. A product that provides an all-in-one procedure. And to be able to do with it a fast and safe collection; as well as to ensure its stabilization and the transport of one-milliliter saliva samples. And all can be done at room temperature, as the product stabilizes the viral DNA and RNA.
This stabilization is possible thanks to REAL VIRAL Stabilization Solution. A product that efficiently inactivates viruses. What solutions for the handling of non-infectious samples, making possible a safe handling and shipment of all these tests.
One of the main advantages of this system is that the samples are kept at room temperature. In the case of DNA, up to one year. Regarding RNA, up to a month. In case they are subjected to low temperatures, the conditions change. And it is that if they are kept between -20 / -80 ° C they can be kept indefinitely.
Advantages of the Real Saliva collection and stabilization system
The main advantages of the sample collection, stabilization and transport system developed by the Real company are the following:
- Allows the handling of samples of one milliliter of saliva.
- Inactivates microorganisms and viruses for easy and safe handling and transport.
- It is a product that allows the isolation of human genomic DNA and viral DNA / RNA. A variety not offered by other similar products on the market.
- As already mentioned, this product allows transport at room temperature. This means eliminating cold infrastructure elements that hinder and retain transportation by requiring more procedures and paperwork.
- Makes it possible to preserve total DNA in samples, including viral DNA, at room temperature. But if a temperature between 4º-25ºC is kept, it will be possible to do so for a whole year.
- In the case of RNA there are very interesting advantages. And it is that it allows total RNA to be preserved, including viral RNA, at room temperature. And if we do it in a cold system (those that are between 4º-25ºC can be kept for a whole month.
- They are systems compatible with most of the DNA and RNA isolation methods found on the market. Although it is best to use REAL products. Both those offered as kits and those sold independently.
- It is a product specially designed for health professionals. And its handling is very simple and intuitive.
So, if you are looking for a collection, stabilization and transport system for any of your saliva samples, at Durviz we have what you need. Do not hesitate, visit our website to expand this information and get in touch with us.
Rapid COVID 19 test from blood, serum or plasma
If you need to do a rapid COVID 19 detection test from blood, serum or plasma samples, at Durviz we have the product you need. It is an exclusive proposal for use in laboratories
Rapid COVID 19 test from blood, serum or plasma
The rapid test that we propose at Durviz is a lateral flow chromatographic immunoassay that allows the qualitative detection of IgG and IgM antibodies against 2019-nCoV. An action that it is capable of carrying out in human blood, serum, plasma or also in a blood sample with a lancet.
This product uses immunochromatography as a detection technique. These types of tests are commonly used for medical diagnosis both for home tests and for use in the laboratory. Hence, it is one of the most requested media by professionals at the present time.
And it is that immunochromatography is one of the most widely used immunodiagnostic techniques today. Its main attractions are: the simplicity and speed of the tests that use this proposal. Hence, with the current need for diagnoses due to the COVID 19 pandemic, there are more and more applications of this technique in the field of tests. Especially since it does not need reagents or additional instrumentation for its operation. Something that does occur in other similar methodologies that are applied to the clinical field.
Advantages of the rapid COVID 19 test
The main advantages of this product are the following:
- It is presented in a strip format, in which the test sample flows along a solid substrate through capillary action.
- This is a combined antibody test that can provide information on the stage of infection of the person being tested. And it is that the detection of COVID-19 IgM antibodies tends to indicate a recent exposure to COVID-19, while the detection of COVID-19 IgG antibodies indicate a later stage of infection.
- The test contains anti-human IgM and anti-human IgG as capture reagent, 2019-nCoV antigen as detection reagent. An IgG reagent is used in the control line system.
- It is a product for exclusive use in laboratories.
To all this must be added, in addition, that IgM provides the first line of defense during viral infections, followed by the generation of adaptive responses of high anity of IgG for long-term immunity and immunological memory.
COVID 19 Rapid Test Features
The main features of the COVID 19 rapid test are as follows:
- Testing should be done immediately after samples have been collected.
- Serum and plasma samples can be stored at 2-8 ° C for up to 7 days. For long-term storage, serum / plasma samples should be kept below -20 ° C. Whole blood collected by venipuncture should be stored at 2-8 ° C if testing will be performed within 2 days of collection.
- Negative results do not rule out SARS-CoV-2 infection. This should be taken into account particularly in those people who have been in contact with the virus. A follow-up test with a molecular diagnosis should be considered to rule out infection in these individuals.
- Results of antibody tests should not be used as the sole basis for making a diagnosis. Neither to exclude a SARS-CoV-2 infection or to report infection status.
So, if you are looking for a system that allows you to perform a rapid test to detect the presence of COVId 19 from blood, serum or plasma, do not hesitate and visit our website. You can buy both this product that we have reviewed in this article, as well as many other accessories. Everything so that you can acquire the best and most reliable results in your laboratory.
Vitromed V-GRAD for sperm separation
If you are looking for products with which you can improve the research you develop in your fertility clinic, such as products with which you can carry out sperm separation, in Durviz we have everything you need.
It does not matter whether the work is carried out in the field of a laboratory or a research ed (clinical, university-research or veterinary in selective breeding). In all of them you can use it with total peace of mind and sure that the results you get will be very reliable. We only have first manufacturer brands, with all the warranty seals and having passed the respective controls of each of them.
Durviz products for sperm separation
In Durviz we distribute a lot of products with which we can carry out the separation of sperm, although one of the most popular is V-Grad from the company Vitromed.
This product is a sterile colloidal suspension of silica particles stabilized with covalently bonded hydrophilic silanes. That is, a density gradient, which allows to separate the seminal sample according to the ability of sperm to pass through different media with different density.
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In this particular case V-Grad is concreted in a kit composed of two glass bottles of 12 milliliters and 40% of 80 milliliters each. V-GRAD is a product that carries endotoxin tested and that has a sperm survival test close to 80% in all cases in which it has been used during its study.
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For better visibility during laboratory handling, this product is colored with phenol red. Which makes it easy to use at all times. It is a product that we can use in the long term, since the validity period of the sample that is handled is 18 months from the date of production.
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Vitromed Quality
The efficacy of Vitromed products is widely accepted in the clinical sector. Hence it is one of the most prestigious of those that exist today. Not all producers can boast the following quality standards:
- As in all the products that we can purchase from the Vitromed brand, these are substances, tools and laboratory products made of medical grade polymers that are manufactured under clean room conditions, ensuring successful assisted reproduction.
- In addition, all of them have been sterilized by radiation according to the ISO standard. They have also been tested for toxicity prior to the release of the product on the market.
- All our laboratory products are tested for MEA and LAL. This is an added guarantee for the quality of the tests to which each of them have been subjected.
- It is a company that develops and manufactures micro tools, needles and many other clinically advanced laboratory articles used at all stages of assisted reproduction techniques (popularly known as ART). Hence, its research and development department includes experienced German scientists, opinion leaders and engineers who work as a team to develop quality IVF products. Minds that progressively output high-quality products that allow important advances in the field of assisted reproduction.
If you are interested or interested in purchasing any of the products that we have in Durviz to carry out the separation of sperm, or any other of the Vitromed brand (we have many in our catalog that you can know online from your own home) contact us. We serve all of Spain and are exclusive distributors of the main companies in the sector.
INTRON: DNA/ARN Virus Purification Kit
Intron is a DNA and RNA extraction kit that is designed for the rapid and sensitive isolation of either of these two substances from a variety of pathogens such as viruses, bacteria, etc.
It’s a versatile product. In fact, it works with all kinds of samples. Hence we can use it with plasma, with fresh or frozen blood (treated with anticoagulants other than heparin), with serum, with other body fluids free of cells and cells or tissues that have been infected with pathogens.
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This DNA and RNA extraction kit is specifically designed to isolate high-quality nucleic acids from a variety of pathogens and samples using low elution and volumes that allow sensitive downstream analysis.
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Once used, RNA or purified DNA is free of proteins and nucleases, thus being suitable for use in subsequent pathogen detection applications.
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The DNA or RNA extraction kit uses the caxotropic salt in the immediately inactive lysis buffer DNasa or RNasa to ensure isolation of any of them intact. This also entails: greater efficiency of lysis (by not requiring additional additives), performing stable DNA or RNA extraction from various pathogenic samples; use a method without phenol and without the need for EtOH precipitation, and to be able to have in our laboratory the possibility of properly performing the extraction of samples in situations as disparate as forensic medicine or the diagnosis of diseases, among others.
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To achieve all this, this extraction kit uses advanced silica gel membrane technology that allows for rapid and effective purification of DNA or RNA without organic extraction or precipitation with ethanol. In addition, the damping conditions are finely adjusted to provide optimal DNA or RNA binding to the spine.
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DNA and RNA extraction Kit Protocol
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The procedure instructions for the extraction kit are very simple; allowing its users to purify DNA or RNA from a wide variety of target sources in as little as 30 minutes. In fact, it is as follows:
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1. Transfer 150 (300) μl of plasma, serum, urine, cell culture supernatant, cell-free fluid or virus infection into tissue or cell in the 1.5 ml microcentrifuge tube.
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If the volume of the sample is less than 150 L, the sample should be set to 150 L with WATER treated with DEPC. If the volume of the sample is higher, 15 ml. centrifugal tube should be used.
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2. Add 300 (600) μl lysis buffer. If the volume changes, vary the quantity.
3. Mix by stirring in vortex for 15 seconds.
4. Incubate at room temperature (15-25 °C) for 10 minutes.
5. Add 300 (600) μl joining buffer and mix gently.
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If the sample volume is greater than 150 He, we should increase the amount of bonding buffer.
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6. Place a rotating column in a 2 ml collection tube provided.
7. Load the lyses into the column and centrifuge at 13,000 rpm for 1 minute.
8. Dispose of the solution in the collection tube and place the column back in the same 2 ml. collection tube.
9. Add 500 μl of wash buffer A, to the column and centrifuge for 1 minute at 13,000 rpm.
10. Dispose of the solution in the collection tube and place the spin column back into the same 2 ml. collection tube.
11. Add 500 μl of wash buffer B to the column and centrifuge for 1 minute at 13,000 rpm.
12. Discard the solution in the collection tube and place the spin column back into the same 2 ml. Centrifuge collection tube for 1 minute at 13,000 rpm.It is important to dry the membrane as residual ethanol may interfere with downstream reactions.
13. Place the column in a 1.5 ml microcentrifuge tube without RNasa (not included), and add 30 – 60 l of elution buffer directly onto the centrifuge column membrane. It is important to dry the membrane as residual ethanol may interfere with downstream reactions. Do not touch the membrane with the tip of the pipette.
14. Incubate at room temperature for 1 minute, then centrifuge for 1 minute at 13,000 rpm.
15. Use 2-5 μl of elued solution as a template for PCR or RT-PCR.
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In case you are interested or interested in purchasing this product, please contact us, Durviz.
Biocomma
Biocomma is a means of transporting and preserving any virus (virus inactivation). A proposal with which it is possible to carry out the collection of samples, avoiding the risk of aerosol infection.
Y is that Biocomma is a solution that allows to inactivate the virus sample in time avoiding infections and protecting the RNA from degradation.
This system can be used for the collection and transport of samples from: clinical influenza, avian influenza, HFMD, measles and other virus samples as well as mycoplasma, ureaplasma, chlamydia and other samples.
Biocomma: Operation
The process we must follow when using Biocomma is as follows:
- Write the information that we consider important regarding the sample on the label. We must do this before we take the shot.
- Depending on the purpose of the analysis, the swab may be used to collect samples from the relevant parts.
- Put the swab in the preservation solution immediately after we have taken the samples. Then you have to break the swab rod and finally close the lid firmly.
- Once the samples have been taken, they must be transported to the laboratory within two working days of collection. The storage temperature should range from 2 to 8 °C. If it cannot be delivered to the laboratory within 48 hours, it must be stored at -70°C or less, and ensure that the collected samples are delivered to the relevant laboratory within 1 week. In any case, we should avoid repeated freezing-thawing cycles.
- If the samples cannot be analyzed within 24 hours of collection, they must be stored at -70°C or lower (if there are no storage conditions at -70°C, they should be temporarily stored in the refrigerator at -20°C).
- Samples used for virus isolation and nucleic acid detection should be processed as soon as possible.
Only by following these steps will we make the results we obtain from our samples as faithful as possible.
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Biocomma application
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Sampling method
There are two sampling methods we will be able to use with Biocomma. The following:
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a) Nasal hisopo. To do this simply gently insert the swab head into the nasal canal, remain for a while, then slowly turn outwards. Take a sample from the other side of the nostril with another sample, immerse the sample head in the preservation solution, break the sample rod and discard the top rod.
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b) Throat torunda. In this case, just take a torunda of the bilateral pharyngeal tonsils and the back wall of the pharynx, submerging the head of the torunda in the preservation solution, break the rod of the torunda and discard the upper rod.
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Warning
It should also be borne in mind that the use of Biocomma should not be carried out in the following cases:
– It is not a product for internal or external use in humans or animals.
– Not to be ingested.
– It’s irritating to the eyes and skin. If it accidentally splashes in your eyes, we should rinse with water.
– We must maintain ventilation during use.
– This product is a colourless, clear liquid. If the properties change, such as yellow, it should not be used
– This product is for in vitro diagnostics only.
– Not to be ingested.
– It’s irritating to the eyes and skin. If it accidentally splashes in your eyes, we should rinse with water.
– We must maintain ventilation during use.
– This product is a colourless, clear liquid. If the properties change, such as yellow, it should not be used
– This product is for in vitro diagnostics only.
– The liquid in the sampling tube is the means for transport, so it is important that it is present and in good condition.
– Waste from the sample collection solution will be sterilized.
– No leak.
– Waste from the sample collection solution will be sterilized.
– No leak.
If you need or want to benefit from the benefits of this product contact us, Durviz.
Company Summer Schedule 2020
Durviz changes its company schedule during july, august and september.
Please keep in mind our company will be closed from 17th to 21st September
Covid-19 quick test for IgG/IgM detection
You can now find covid-19 quick test for IgG/IgM detection in Durviz. An immuno lateral flow chromatographic assay for the qualitative detection of IgG and IgM antibodies against 2019-nCoV in human blood, serum, plasma or also in a lancet blood sample. This puts at our fingertips an effective method for rapid diagnosis of COVID-19 infection in the person undergoing the test.
And all this doing a comprehensive analysis of the composition of the elements in the blood. So the detection of COVID-19 IgM antibodies tends to indicate recent exposure to COVID-19, while detection of COVID-19 IgG antibodies indicate a later stage of infection. Information needed to perform epidemiological studies, as well as the degree of involvement the disease had on the person.
And it is widely accepted that IgM provides the first line of defense during viral infections, followed by the generation of IgG’s high-anity adaptive responses for long-term immunity and immune memory.
Covid-19 quick test: IgM efficiency
Immunochromatography is one of the most relevant immunodiagnostic techniques out there. Its main advantages are: the simplicity and speed of the test.
Y is that more and more applications are being made of this technique, both in the field of tests, because no reagents or additional instrumentation are necessary; and in the clinical field.
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This action can be performed using a simple device developed to detect the presence (or absence) of a target compound in the sample (the matrix). Element that makes up this test. It consists of two components: an IgG and an IgM, which allow to detect the presence of IgG and IgM antibodies independently.
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Its operation is simple and very effective since the region of the IgG detection line is coated by human anti-IgG, and when depositing the sample in the analysis area, the possible IgG anti 2019-nCoV present, react with colored particles coated with antigens of 2019-nCoV present in the cassette. The mixture migrates through the membrane by capillary and reacts with human anti-IgG antibodies in the test region reserved for IgG detection. If the sample contains IgG antibodies for 2019-nCoV, a colored line appears in the designated region of IgG as a result of this antigen-antibody bonding reaction.
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Similarly, in the IgM detection line, human anti-IgM antibodies are attached, in the case of a positive sample for IgM from 2019-nCoV, the complex formed together with the 2019-nCoV antigens reacts with the anti-IgM antibodies and a colored line appears in the corresponding region.
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If the sample contains neither IgG nor IgM, no lines will appear in the test areas for IgG or IgM, indicating a negative result. However, as a check to ensure that the sample volume used is sufficient and that the procedure has been successful, a third line appears in the control area.
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These types of tests are used for medical diagnosis and home testing, as well as in the lab.
Covid-19 quick test
A reliable and quality test
Given the importance of results in people’s lives and in the safety of their environments, special attention has been paid to test controls.
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In fact, internal controls are included in the test. This is displayed on a colored line appearing in the control region (C) composing the internal control of the procedure. This ensures that the technique carried out is correct and that the sample volume used is sufficient. Control standards are not included in the kit, however, it is recommended to test with negative and positive controls as indicated by good laboratory practices to verify the test result.
Centrifuges for concentration techniques
If you are looking for a device with which you can perform concentrations in your laboratory, and by extension to be able to formulate adequate diagnoses with little margin of error, the concentration centrifuges of the Real brand, will surprise you pleasantly.
These are two models of centrifuges (BDT4 and BDT4-2) that will allow you to perform numerous actions with which you will gain time and effort in the daily tasks of your laboratory.
To get to know them in depth, in Durviz we have written this article with which we want to help you choose the model that suits you best.
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Models of concentration centrifuges
Centrifugal to develop different concentration techniques are devices that allow you to perform laboratory tests in a short time and with high reliability.
The BTD4 model is a small concentration centrifuge, specially designed for human diagnostics. It is portable and its operation is very simple. It is a tube-compatible device of up to 20 ml of the Real brand and reaches a speed of 4000 revolutions per minute. Includes an 8-vial angular rotor of the same brand.
Works thanks to a maintenance-free induction motor. In addition, this engine is suspended on rubber parts that reduces vibrations that may occur during operation.
REAL Centrifuge Model BTD4
The BTD4-2 model is a concentration centrifuge designed for routine medical analysis of hospitals. It is a model that has a wide variety of accessories, which make them also compatible with the analysis work that is carried out in research processes.
Is a centrifuge compatible with tubes with capacity up to 50 milliliters. Like the other model of the brand, this device also reaches a speed of 4000 revolutions per minute.
It has a 50 milliliter Real Midi sistem six-pipe angular rotor, or for an oscillating rotor of up to 8 15 milliliter Real Midi System tubes. Sus main physical characteristics are as follows:
- It has an exterior metal structure.
- It has a series of rubber supports that reduce vibrations that can arise when in operation.
- It has a series of gas springs to prevent the door from falling.
- You have an emergency release of the lid lock.
- It has a safety lock on the lid to prevent it from opening during centrifugation.
- Its operation is extraordinarily quiet.
- It has a countdown timer that marks the times of each process.
- Allows the recovery of the data of the last set parameters.
- Performs a self-diagnosis of errors in case they appear.
REAL Centrifuge Model BTD4-2
Both concentration centrifuges are compatible with the Real brand Mini and Midi Sistem systems.
All these products allow to make a higher performance to the systems of obtaining, preserving and analyzing all kinds of human or animal samples. Especially those related to the tests of intestinal parasites from faecal samples, but not exclusively to them.
At Durviz Laboratories we put them at your fingertips so you can enjoy the features of both models.
To buy them you only have to enter our website and purchase them, once you have registered as a customer. We will take care of sending them to your home or the home you indicate, with all the guarantees and care that each product requires. And always at the best price. Remember, in this sense, that we are official and authorized distributors of the main brands manufacturers of laboratory products. Something that reinforces the way we work and that encourages us to continue to grow in the sector, where we already occupy a prominent place.
Online seminars, latest news in diagnosis and research
We keep you informed of the latest developments in the areas of diagnosis and research through online seminars and meetings
Tapestri Designer 2.0, New Improvements to scDNA-seq Custom Design
How can you use an AI powered engine to design custom single-cell DNA panels in minutes? Mission Bio has just launched Tapestri Designer 2.0 and will walk you through the steps involved with creating your custom panel.
New features include:
Entire human and mouse genome. Great for early phase drug development.
Includes exons and introns. Perfect for CRISPR applications.
Join us to unlock the power of single-cell DNA sequencing for your research needs and receive a special offer code for attending.
2 jun 2020 06:30 PM en Madrid
