Posts

DNA extraction kit from saliva at Durviz

in ,

Saliva is a substance that can give us a lot of information about a person’s state of health. At Durviz we know how interesting and reliable the data that can be extracted from a person’s saliva is, which is why we have products that facilitate its analysis. It is very important to have first-class products that help us to preserve the saliva sample without any contamination..

For this reason, in our virtual store we have products such as the Real Saliva Collection and Transport, one of the most popular on the market. This kit provides a fast and safe all-in-one method for the collection, stabilization and transport of saliva samples at room temperature.

Its main characteristics are the following:

  • Easy collection, transport and processing of saliva samples.
  • Painless collection, and non-invasive (as opposed to other systems where samples are taken from patients).
  • Allows saliva samples to be stored for 2 years.
  • The saliva kept in this kit is preserved and ready to be sent.
  • It allows the subsequent isolation of DNA making it an element of excellent quality and integrity.

The operation of this kit is very simple and requires few steps, which speeds up the work in the laboratory. The first thing to do is to collect the saliva samples. This is done by simply spitting into the collection funnel that has been assembled with a collection tube. This eliminates almost 100% of the possibility of contamination.

After collecting 2 millilitres of saliva (container capacity), the content of the saliva preservation solution is added and mixed with the extracted saliva. Finally, the saliva collection tube is sent to the laboratory for further isolation and DNA analysis.

This is usually recommended with the Real Pure saliva kit, which follows the same protocol and
that you get unbeatable results. The saliva samples are kept stably for more than one year at room temperature and more than 2 years if these same samples remain within the following temperature range: -20ºC or -80ºC.

DNA-extraction-kit-from-saliva-at-Durviz

For best performance, the manufacturer also recommends the following for the
when saliva samples are collected and preserved:

1. It is advisable not to eat, chew gum, drink or smoke during the 30 minutes before the saliva sample is taken.
2. It is advisable to wash out the mouth with water and wait for 10 minutes.
3. It is better if the patient rubs his cheeks and the palate with his tongue before collecting the sample. This gesture is sufficient to stimulate the secretion of saliva.
4. Before starting to collect the sample, it is convenient to place the funnel in the saliva collection tube. Then just spit the saliva into the funnel until the liquid (not including the bubbles) reaches the 2 milliliter line in the collection tube. If there are excess bubbles, they should be removed by gently tapping the tube on a hard surface.
5. Remove the blue cap from the saliva preservation solution.
6. Add the saliva-conserving solution to the collection tube.
7. Close the saliva collection tube tightly to avoid accidents or contamination.
8. Shake for 15 seconds to thoroughly mix the products with the saliva.
9. Once this procedure is done, the saliva sample is now preserved and ready to be stored, shipped or processed.

For all these reasons, this new model of Saliva Collection Kit is one of the most popular on the market. And one of the most popular among laboratory professionals.

Kit Real de microbioma fecal

Real Kit of Faecal Microbiomes

in

Do you want to have the latest on the market to make a faecal microbiome? Do you want to work with maximum warranty products? Do not hesitate and acquire the Real brand Kit to carry this kind of tests. A way to ensure no failure on the obtained results.  

In fact, it was developed to be applied on a wide range of  areas like for example: microbiome analysis, RFLP analysis, mutations or even the need to identify pathogens. 

Faecal micobiome Real Kit Features

A microbiome  is a group of microorganisms, generally simbiotic, that are part of every human, animal or plant. In case of the human being we are talking about thousands of microorganisms that live with us every day, in and out of our body. That is, of microbial communities, genes and metabolites that colonize our organism. 

In order to study them and get to understand them as a support for studying a disease and its behaviour or how to treat it, several tests are carried. One of this tests is the human faecal microbiome study. A test also carried on animals that helps to prevent a wide range of issues and get to know a great variety of diseases.

At present, this test is being considered as essential on the development of really important investigations, specially on those realtes with predictions of patological states as obesity, cancer or cardiobascular diseases, neurodegenerative or respiratory, where professionals are making significant progress.

But also to relate diseases with some of our life aspects, in the begginig more secondary aspects. For example, Finding out if certain oral cavity diseases such as gingivitis are related to preterm births or low weight on birth. Eventhough at present time its applications are being developed for more areas and they will continue expanding on this matter for the following years.

 To carry this faecal microbiome study, it is necessary to purify the microbial dna, otherwise the study of it could not be carried out from different faecal samples. An aspect that is done without problems with the use of the Kit that the Real brand has developed in this sense. A Kit owning the following features: 

 

  1. In an optimal way, it develops a lisis method with a combination of: heat, quimical and mechanical lisis using specific beads. What it pretends to obtain is the perfect homogeneization of the sample. What is translated into, an isolation of the dna of yeasts, fungi, Gram-negative and Gram-positive bacteria. 
  2. Its a simple and easy process that uses Microspin columns. 
  3. It has been design in order to obtain an efficient and fast micronbiome dna purification from several faecal samples. 
  4. No phenol nor choloform is used on its operation; not for extraction neither for ethanol precipitation, what means a preservation of the sample to avoid biased results. 
  5. It has a great capacity. This kit allows us to work with up to 200mg of human faeces. A different amount in case of animal samples, where it is recommended the reduction of  sample size which is between 80 and 100 mg. 

Kit Real de microbioma fecal

For all of the above, in Durviz, we offer the Real brand products to carry the study of faecal microbiomes. There is no other way to obtain the best results than using the best products from the best manufacturers. All of them can be found in Durviz in a simple click.