Tag Archive for: genotype

From now on people and companies who wish to purchase MissionBio branded products in Spain can do so through the Durviz. website. This entity will be responsible for, from now on, the exclusive distribution, for our country, of the products of the firm MissionBio. A highly valued brand in the field of single cell DNA analysis using Tapestri technology.

Tapestri technology is very useful for the study of disease behavior since in their development these infections mutate and it is necessary to know how they do it to improve in applications, their treatment and their resistance.

 

To achieve this, MissionBio’s Tapestri system performs the following actions:

1. Breaking mass limitations.

At this time, Mission Bio’s Tapestri system makes the average reading of conventional mass sequencing; average reading of conventional mass sequencing is performed; which means it loses the underlying genetic diversity in cell populations. To improve patient stratification, therapy selection and disease monitoring, we need information on the occurrence of mutations within each cell and this is what MissionBio offers us with this system.

 

2. The industry’s first scalable, customizable single-celled DNA analysis platform.

Tapestri is presented as the industry’s first scalable and customizable single-celled DNA analysis platform. It should be borne in mind, at this point, that the Mission Bio Tapestri platform was developed to help advance precision medicine, as its application allowed for accelerated and accessible detection of genomic variability within and between cell populations.To do this, taking advantage of the patented microfluidic droplets, the platform unlocks access to DNA at the level of individual cells with a novel two-step protease workflow. Actions whereby this methodology provides flexibility for additional applications and customization capability in our work.

In this way, the Mission Bio Tapestri platform enables the advancement of precision medicine by enabling the rapid and accessible detection of genomic variability within and between mobile populations. Something that happens by taking advantage of the patented microfluidic droplet, the platform unlocks access to DNA at the level of individual cells with a novel two-step protection workflow.

With all this, this methodology provides flexibility for additional applications and customization capability.

And this platform has the following features:

• SNV directed and accurate and so-called independent variants.
• Single cell DNA performance up to 10,000 cells.
• Simple workflow.
• Easy-to-use bioinformatics software.
• Fully customizable content.

How Tapestri platform works

The Tapestri instrument divides thousands of cells into individual droplets, which act as mini reaction tubes that allow thousands of individual reactions simultaneously.

In this way, cell lysis and protease digestion then continue outside the instrument allowing access to DNA and proteins for downstream amplification.

In detail, the steps in which the work of this MissionBio system is developed are as follows:

Step 1: The Tapestri instrument divides the cells into droplets along with a mixture of protease enzymes. Cell lysis and protease digestion are then performed, outside the instrument, allowing access to DNA for downstream amplification.

Step 2: Cellular lysate is combined with pearls, primers and reagents with barcode. Specific regions of interest are amplified by labeling each enlargement with a unique cell barcode, preserving the cell’s identity and mutation profile.

Step 3: After running your sequencer, the system continues to use a subsequent analysis and visualization software.To do this, Mission Bio provides dedicated bioinformatics support to help you discover biological and clinical knowledge

So, if you want to improve research based on single-cell DNA analysis, trust MisssionBio systems. In this way you can obtain the most reliable results and be able to develop very efficient strategies against different diseases.